AAPS Journal is excited to announce the publication of a study on "Application of DOE to ELISA Robustness and Ruggedness Assessment".
This recent study demonstrates how applying Design of Experiments (DOE) can efficiently evaluate robustness and ruggedness factors in complex assays like immunoassays, where traditional testing can be experimentally challenging. By using a 16-run Resolution III design, the team systematically assessed 15 factors, identified key sources of assay variability, and reduced confounding through strategic data analysis, offering a practical approach to optimize ELISA performance with limited experimental resources.
Read the full article and learn more about the study's findings at: https://link.springer.com/article/10.1208/s12248-025-01048-3
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Pankajini Mallick Ph.D.
Sr. Principal Scientist, Preclinical Pharmacokinetics | Modeling and Simulation
San Diego CA
Disclaimer: Opinions expressed are solely my own and do not express the views or opinions of my employer.
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