Bioanalytical Community

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

Judging by the collection times, your protein is not an antibody. Therefore, it's really rather hard to tell how it's going to respond to the duration of processing. We once spiked an antibody into whole blood and let it sit in an SST tube overnight at room temperature before converting to serum. There was no haemolysis and the antibody recovered nicely after such abusive treatment. But antibodies are very sturdy in serum and the blood came from a healthy volunteer. Without knowing what your protein is/does and where the blood came from (animals, healthy or diseased human subjects?) there is no way of telling how it's going to behave sitting on the clot in an SST tube.

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

Judging by the collection times, your protein is not an antibody. Therefore, it's really rather hard to tell how it's going to respond to the duration of processing. We once spiked an antibody into whole blood and let it sit in an SST tube overnight at room temperature before converting to serum. There was no haemolysis and the antibody recovered nicely after such abusive treatment. But antibodies are very sturdy in serum and the blood came from a healthy volunteer. Without knowing what your protein is/does and where the blood came from (animals, healthy or diseased human subjects?) there is no way of telling how it's going to behave sitting on the clot in an SST tube.

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

Judging by the collection times, your protein is not an antibody. Therefore, it's really rather hard to tell how it's going to respond to the duration of processing. We once spiked an antibody into whole blood and let it sit in an SST tube overnight at room temperature before converting to serum. There was no haemolysis and the antibody recovered nicely after such abusive treatment. But antibodies are very sturdy in serum and the blood came from a healthy volunteer. Without knowing what your protein is/does and where the blood came from (animals, healthy or diseased human subjects?) there is no way of telling how it's going to behave sitting on the clot in an SST tube.

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

Hi Everybody,

               Joleen makes a very good point – it's hard to accurately spike analyte into whole blood in SST. We are fortunate enough to have blood collection on site, and we had access to freshly collected (still warm!) blood in SST. The SST tubes were still under vacuum so spiking was done with a syringe; you puncture the septum and the vacuum in the tube "sucks in" the contents of the syringe. Our experiments were of "look/see" nature and much more work would be required to bring them up to validation standards. Our protein was a monoclonal antibody, so we didn't have to worry about binding to plasma proteins or penetrating cells. For proteins that do, designing a good experiment would take more thought. Good luck!

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!

I do not  know what kind of protein it is but should  try Dried blood spot technique 

We have a biologic (protein) that has collection timepoints at 16 and 20 hrs. on D7 for terminal half-life determination.  We will be able to draw the whole blood however there would be no staff to process this immediately into serum at the time of collection. The blood is collected in SST tubes and allow to clot for 30 minutes, centrifuged and aliquots are made before freezing.  However, at these timepoints the SST tube will be draw but not processed immediately.  Any prior experience with other large molecule studies on what to do at these later timepoints when blood cannot be processed immediately? How is the blood handled?

Thank you!