In drug development and neurological research, the permeability of substances across the blood-brain barrier (BBB) is crucial for predicting their efficacy and safety in vivo. Brain endothelial cell uptake studies are commonly employed in vitro to assess BBB permeability. However, the reliability of these studies in predicting in vivo brain uptake remains uncertain.
Recent advancements have introduced more complex in vitro models, including co-cultures and tri-cultures incorporating brain endothelial cells along with astrocytes and neurons, aiming to better mimic the neurovascular unit and improve predictive capabilities. How do these advanced in vitro models compare to traditional brain endothelial cell uptake studies in predicting BBB permeability and in vivo brain uptake? Additionally, if present what types of in silico predictions are available to reflect the accuracy of these permeability predictions?
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Sejal Sharma
Research Assitant
Texas Tech University Health Sciences Center
Amarillo TX
[email protected]Disclaimer: Opinions expressed are solely my own and do not express the views or opinions of my employer.
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